Topic

No. of hours

Lecture

practical

(1)Introduction

(2)Fundamental characters of viruses

1

1

(3) General Properties of viruses

A. Physical properties of viruses.

B. Chemical properties of viruses.

2

2

2

2

(4) Viral Haemagglutination

1

1

(5) Virus cell relationships (virus multiplication)

2

2

(6) Pathogenesis of viral infection

2

2

(7) Interference phenomenona

1

1

(8) Viral immunity

1½

3

(9) Viral vaccines

1½

(10) Effect of physical & chemical agents on viruses

1

1

(11) General scheme for viral isolation & identification

3

3

(12) lab safety

3

3

(13) sampling

• Collection
• Preservation
• preparation

6

6

(14) lab animal

• advantage
• disadvantage
• route of inoculation
• hyper immune serum
• monoclonal antibodies

6

6

(15) fertile egg

• advantage
• disadvantage
• specifications
• structure
• route of inoculation
• harvestation
• signs and factors affecting 

12

12

(16) tissue culture

• advantage
• disadvantage
• equipments
• tissue culture media and solution
• types of cells
• basic requirements for growth of cells
• preparation of primary culture
• subculture of cells
• preservation of cell culture
• inoculation of cell culture
• CPE
• Harvestation of inoculated cell culture 

15

15

total

60

15

45